Rubik's cube

Fantasy organisms between plants, animals, humans, bacteria & viruses

Fantasy organisms between plants, animals, humans, bacteria & viruses

Iko Koevoets  — Biologist
click » here « to watch movie (Dutch spoken)

—set-up 8 petri dishes with actual Arabidopsis thaliana NaCl plant experiments
—screen with 8 live petri dishes with Arabidopsis thaliana NaCl plant experiments: rhizomatique.org
—CRISPR kit gene editing (The Odin)
—tower block petri dishes lighted by fluorescent tubes
​​​​—amazing plants defying boundaries between plants, animals and humans
—girls drafting fantasy organisms
—heaps of salt... How do plants sense salt? (Christa's ERC: Surviving salinity: How do plants sense Na+?) (ERC »Sense2SurviveSalt«)

                                    “Fascination of Plants Day” 2017 (link)

                                    The Green Machine

& Research Group Christa Testerink: Ayodeji Deolu-Ajayi & Dorota Kawa & Ruud Korver & Daan Mangé & Jessica Meyer & Yanxia Zhang

Arabidopsis thaliana

Arabidopsis thaliana propagation in the Greenhouse of the Faculty of Science

Greenhouse of the Faculty of Science, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam

Arabidopsis thaliana

The annual wild weed species Arabidopsis thaliana is a model organism not only for molecular biology but also for ecological and evolutionary genetics, and hence, revealing the geographical structure of its genetic variation has become of paramount relevance.
Furthermore, identification of genetic structure is relevant to mapping the causal genes responsible for the natural variation of adaptive traits by genomewide association analysis.
The genome of the flowering plant Arabidopsis thaliana has five chromosomes
the largest, chromosome 1, in two contigs of around 14.2 and 14.6 megabases
Chromosome 1 contains about 300 gene families with clustered duplications
XXX genes … (to name a few: … ) …
xx genotypes
500 populations across its world distribution

Found objects, Laminar Air Flow Swammerdam Institute for Life Sciences University of Amsterdam

Thought I had found Wild-type Arabidopsis thaliana, but did not

Brilliant Green

seeds start shooting… even in tube, even in the dark, even left in dark at 3 ºC for almost a month (sterilized and put in agar/medium Aug. 26th 2016) … talking about adaptive capacity

Arabidopsis (Col-0) seedlings have grown into the agar towards the light (black setup container holding the plates prevents light from above and back, and seedlings orientate towards the light tubes).


Phototropism
ˌfəʊtə(ʊ)ˈtrəʊpɪz(ə)m,fəʊˈtɒtrəˌpɪz(ə)m/
noun
BIOLOGY

the orientation of a plant or other organism in response to light, either towards the source of light (positive phototropism) or away from it (negative phototropism).

Was ist der Mensch?

(1) What can I know?

(2) What ought I to do?

(3) What may I hope for?

(4) What is man?

genotype: unknown (lettering bleached by sunlight)

genotype: unknown (lettering bleached by sunlight)

treatment: grown in studio light

… follows its “aparallel evolution” through to the end.
The wisdom of the plants: even with something else
— with the wind, an animal, human beings…
“Drunkenness as a triumphant irruption of the plant in us.”

Flowering Above and Below

genotype: SUR1-1
medium: ½MS, 0mM NaCl
date sown: Aug. 05 2016
date image: Sep. 23, 2016
age: 7 weeks old

genotype: Col-0
medium: ½MS, 0mM NaCl
date sown: Aug. 05 2016
date image: Sep. 23, 2016
age: 7 weeks old

Run 6 Petri Dish: 1
⚘ : Mutant 2 (Arabidopsis thaliana)
▢ Medium : Gradient ½MS + Sucrose / 200 mM Salt

Run 6 Petri Dish: 2
⚘ : Col-0 (Arabidopsis thaliana)
▢ Medium : 125 NaCl

Run 6 Petri Dish: 3
⚘ : An-1 (Arabidopsis thaliana)
▢ Medium: Control (½ MS)

Run 6 Petri Dish: 4
⚘ : SUR1.1 (Arabidopsis thaliana)
and Agrobacterium Tumefaciens strain GV3101
▢ Medium : Control (½ MS)

Charles Darwin's “Tree of Life” sketch
Run 6 Petri Dish: 5
⚘ : Col-0 and LRI (AT)
▢ Medium : Control (½MS)

Run 6 Petri Dish: 6
⚘ : Col-0 (Arabidopsis thaliana) and fungi
▢ Medium : Control (½MS)

Run 6 Petri Dish: 7
⚘ : Col-0 (Arabidopsis thaliana) and other species
▢ Medium : 150mM Sorbitol

Run 6 Petri Dish: 3
⚘ : Col-0 (Arabidopsis thaliana)
▢ Medium : Control (½MS)

Salt Gradient / Halotropism

Preparing Salt Gradient

Sterilise glass cutting tool in alcohol (70% EtOH)

Take out glass cutter and let dry up

One might dry up EtOH with flame, however be careful not to break the glass

Cut the diagonal and scoop out the 0mM agar

Throw left over 0mM (control) agar in a bag (prepare to have installed ;)

Make sure to carve a diagonal & neat, straight line

Results half way. Remember to make some extra plates

Next step: add 200mM Salt (NaCl) in the carved out triangle

Now this is quite a precise job as we don’t want elevations in the changeover

Result: Salt Gradient 0mM to 200mM NaCl

Next time we’ll do the diagonal the other way around

The Nottingham Arabidopsis Stock Centre (NASC) provides seed and information resources to the International Arabidopsis Genome Programme and the wider research community

First batch arrived, in alphabetical order:

N76433 Altai-5
N8068 Berkeley
N75867 Brock-1
N799234 IP-Doñ-0
N76950 Istisu-5

and on their way:

N6730 Gu-0
N77369 Tnz-1

Root Image Time Elapse (RITE) engineered and installed November 1st 2016 by Daan, Johan and Gerrit