“That's a fool's experiment. But I love fool's experiments. I am always making them.” — Charles Darwin (1862)
Thursday, May 18, 2017 06:16 AM
—set-up 8 petri dishes with actual Arabidopsis thaliana NaCl plant experiments
—screen with 8 live petri dishes with Arabidopsis thaliana NaCl plant experiments: rhizomatique.org
—CRISPR kit gene editing (The Odin)
—tower block petri dishes lighted by fluorescent tubes
—amazing plants defying boundaries between plants, animals and humans
—girls drafting fantasy organisms
—heaps of salt... How do plants sense salt? (Christa's ERC: Surviving salinity: How do plants sense Na+?) (ERC »Sense2SurviveSalt«)
Wall setup ready for “Fascination of Plants Day” 2017
Arabidopsis thaliana propagation in the Greenhouse of the Faculty of Science
Greenhouse of the Faculty of Science, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam
Arabidopsis thaliana
The annual wild weed species Arabidopsis thaliana is a model organism not only for molecular biology but also for ecological and evolutionary genetics, and hence, revealing the geographical structure of its genetic variation has become of paramount relevance.
Furthermore, identification of genetic structure is relevant to mapping the causal genes responsible for the natural variation of adaptive traits by genomewide association analysis.
The genome of the flowering plant Arabidopsis thaliana has five chromosomes
the largest, chromosome 1, in two contigs of around 14.2 and 14.6 megabases
Chromosome 1 contains about 300 gene families with clustered duplications
XXX genes … (to name a few: … ) …
xx genotypes
500 populations across its world distribution
Meanwhile, Out in the Fields
Thursday, August 18, 2016 11:07 AM
Seed collection in Butterfly Garden Piet Oudolf in Utrecht, The Netherlands
photo: macha
Found in the Flow (Objet Trouvé)
Friday, August 5, 2016 11:31 AM
Found objects, Laminar Air Flow Swammerdam Institute for Life Sciences University of Amsterdam
ACGT
Friday, July 29, 2016 11:17 AM
Base pairs
Attached to each sugar ring is a nucleotide base, one of the four bases Adenine (A), Guanine (G), Cytosine (C), and Thymine (T).
??
Attached to each sugar ring is a nucleotide base, one of the four bases Adenine (A), Guanine (G), Cytosine (C), and Thymine (T).
Working on the visualisation (hopefully with the help of Ann Ellis and Bette West) as I didn't get it right yet :)
Searching for Arabidopsis thaliana — the Weed in the Wild
Monday, July 11, 2016 03:10 PM
Thought I had found Wild-type Arabidopsis thaliana, but did not
Eriophorum
Sunday, July 10, 2016 01:18 PM
Eriophorum (single spike wool-grass), a rhizomatous sedge. The seed heads are covered in a fluffy mass of wool which are carried on the wind to aid dispersal. High Fens Eifel Nature Park (cross-border region Hoge Venen (Belgium) Eifel Germany)
Brilliant Green
Friday, July 8, 2016 04:09 PM
Brilliant Green
seeds start shooting… even in tube, even in the dark, even left in dark at 3 ºC for almost a month (sterilized and put in agar/medium Aug. 26th 2016) … talking about adaptive capacity
Phototropism
Thursday, July 7, 2016 05:41 PM
Arabidopsis (Col-0) seedlings have grown into the agar towards the light (black setup container holding the plates prevents light from above and back, and seedlings orientate towards the light tubes).
the orientation of a plant or other organism in response to light, either towards the source of light (positive phototropism) or away from it (negative phototropism).
Electrophoresis
Friday, May 27, 2016 05:11 PM
Electrophoresis
DNA electrophoresis is a method used to sort DNA molecules by length. Pieces of DNA are suspended in a tray of gel and subjected to an electric field, which causes them to migrate toward one end of the tray.
Electrophoresis Results
Tuesday, September 27, 2016 02:30 PM
Multichannel Pipetting
Tuesday, September 27, 2016 04:30 PM
Fundamental Research, Fundamental Questions
Friday, November 18, 2016 04:00 PM
Was ist der Mensch?
(1) What can I know?
(2) What ought I to do?
(3) What may I hope for?
(4) What is man?
Waking up to a New World Order
Wednesday, November 9, 2016 05:00 AM
Waking up to a New World Order, Wednesday November 09 2016 5AM
Graceful Root Architecture
Thursday, March 17, 2016 03:00 PM
genotype: Col-0
medium: -SUC, 75 NaCl
A—Parallel Evolution
Thursday, March 17, 2016 03:00 PM
genotype: unknown (lettering bleached by sunlight)
genotype: unknown (lettering bleached by sunlight)
treatment: grown in studio light
… follows its “aparallel evolution” through to the end.
The wisdom of the plants: even with something else
— with the wind, an animal, human beings…
“Drunkenness as a triumphant irruption of the plant in us.”
*
Sunday, October 2, 2016 09:49 AM
Flowering Above and Below
genotype: SUR1-1
medium: ½MS, 0mM NaCl
date sown: Aug. 05 2016
date image: Sep. 23, 2016
age: 7 weeks old
Flowering Above and Below
Sunday, October 2, 2016 09:49 AM
genotype: Col-0
medium: ½MS, 0mM NaCl
date sown: Aug. 05 2016
date image: Sep. 23, 2016
age: 7 weeks old
Halotropism
Friday, August 5, 2016 05:47 PM
Halotropism
halotropism is a response of plant roots to avoid a saline environment
genotype: Col-0
medium: ½MS, 125 NaCl
Halotropism
Friday, August 5, 2016 05:47 PM
Arabidopsis thaliana — Mutant 2
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 1
⚘ : Mutant 2 (Arabidopsis thaliana)
▢ Medium : Gradient ½MS + Sucrose / 200 mM Salt
Arabidopsis thaliana — Col-0
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 2
⚘ : Col-0 (Arabidopsis thaliana)
▢ Medium : 125 NaCl
Arabidopsis thaliana — An-1
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 3
⚘ : An-1 (Arabidopsis thaliana)
▢ Medium: Control (½ MS)
Arabidopsis thaliana — SUR1.1 and Agrobacterium Tumefaciens strain GV3101
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 4
⚘ : SUR1.1 (Arabidopsis thaliana)
and Agrobacterium Tumefaciens strain GV3101
▢ Medium : Control (½ MS)
Charles Darwin's Tree of Life sketch
Saturday, December 31, 2016 01:00 AM
Charles Darwin's “Tree of Life” sketch
Run 6 Petri Dish: 5
⚘ : Col-0 and LRI (AT)
▢ Medium : Control (½MS)
Arabidopsis thaliana and Fungi
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 6
⚘ : Col-0 (Arabidopsis thaliana) and fungi
▢ Medium : Control (½MS)
Arabidopsis thaliana and Other Species and Sorbitol
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 7
⚘ : Col-0 (Arabidopsis thaliana) and other species
▢ Medium : 150mM Sorbitol
On the Origin of Species
Saturday, December 31, 2016 01:00 AM
Run 6 Petri Dish: 3
⚘ : Col-0 (Arabidopsis thaliana)
▢ Medium : Control (½MS)
Tutorial: Salt Gradient / Halotropism
Friday, September 2, 2016 11:56 AM
Salt Gradient / Halotropism
Preparing Salt Gradient
Sterilise glass cutting tool in alcohol (70% EtOH)
Take out glass cutter and let dry up
One might dry up EtOH with flame, however be careful not to break the glass
Cut the diagonal and scoop out the 0mM agar
Throw left over 0mM (control) agar in a bag (prepare to have installed ;)
Make sure to carve a diagonal & neat, straight line
Results half way. Remember to make some extra plates
Next step: add 200mM Salt (NaCl) in the carved out triangle
Now this is quite a precise job as we don’t want elevations in the changeover
Result: Salt Gradient 0mM to 200mM NaCl
Next time we’ll do the diagonal the other way around
NASC Order
Sunday, October 16, 2016 11:49 AM
The Nottingham Arabidopsis Stock Centre (NASC) provides seed and information resources to the International Arabidopsis Genome Programme and the wider research community